Abstract
Spermatozoa from bovine ejaculates and cauda epididymidis were incubated with either tritiated 17β-hydroxy-5α-androstane-3-one (DHT) or 5α-androstane-3α,17β-diol (3α-diol). Examination of the medium incubations demonstrated metabolic conversion of both DHT and 3α-diol when these steroids were incubated with ejaculated sperm. In addition to this interconversion, the following metabolites were identified; 5α-androstane-3β,17β-diol,(3β-diol), androsterone and 5α-androstane-3,17-dione (5α-A-dione). Incubations with cauda spermatozoa showed similar metabolic patterns. Androgen binding was exhibited by both sperm types. Examination of the washed cauda sperm pellet, following incubations with 3α-diol showed that the incubated steroid was the most abundantly bound. DHT and 5α-androst-16-en-3α-ol (Δ 16-3α-ol) were also detected. The major part of the radioactivity bound in the sperm pellet was identified as DHT when this steroid was used as the substrate; the remaining radioactivity consisted of 3α-diol and Δ 16-3α-ol. Investigations of ejaculated sperm pellets gave similar results apart from the additional identification of 5α-androst-16-en-3-one (Δ 16-3-one) and 5α-androst-16-en-3β-ol (Δ 16-3β-ol (Δ 16-3β-ol).
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