Uptake and Cellular Effects of Polymethylmethacrylate on Human Cell Lines

Abstract

The usage of plastic and its decomposition products leads to their ubiquitous distribution, resulting in their uptake by all living beings, including humans. Polymethylmethacrylate (PMMA) is known as a biocompatible polymer and is used widely in medicine and dentistry, although recent findings have shown its induction of oxidative stress within cells. Worryingly, hardly any data exist investigating the uptake of PMMA particles by cells, the potential effects of these particles on cells and cell signaling pathways and their contributing factors. We assessed the uptake of PMMA beads via confocal microscopy after their incubation with HEK293, A549 and MRC5 cells. Through cell staining, we localized multiple PMMA beads within the cytosol of cells. No alterations regarding cell growth, cell morphology or cell division were found, implying no short-term toxicity towards human cells. Using a cAMP response element binding protein (CREB)-mediated reporter assay, we assessed whether internalized PMMA nanobeads alter cell signaling pathways after stimulation of the cells. CREB was chosen as a well-described transcription factor involved in various cellular processes. Our data led to the assumption that PMMA nano- and microbeads are internalized via endocytosis and end up in lysosomes within the cell cytosol. We concluded that differences regarding the surface composition of the PMMA nanobeads affect their potential to alter cell signaling. These findings emphasize the key role the surface composition plays regarding microplastics and their risks for human health, whereas the usage of medical-grade PMMA remains safe.