Abstract
Previous reports have shown that adenovirus recruits nucleolar protein upstream-binding factor (UBF) into adenovirus DNA replication centres. Here, we report that despite having a different mode of viral DNA replication, herpes simplex virus type 1 (HSV-1) also recruits UBF into viral DNA replication centres. Moreover, as with adenovirus, enhanced green fluorescent protein-tagged fusion proteins of UBF inhibit viral DNA replication. We propose that UBF is recruited to the replication compartments to aid replication of HSV-1 DNA. In addition, this is a further example of the role of nucleolar components in viral life cycles.
Highlights
Previous reports have shown that adenovirus recruits nucleolar protein upstream-binding factor (UBF) into adenovirus DNA replication centres
We propose that UBF is recruited to the replication compartments to aid replication of herpes simplex virus type 1 (HSV-1) DNA
UBF is normally associated with rDNA at the rRNA promoters and plays a role in the recruitment of RNA pol I to rDNA, which influences the rate of rRNA synthesis (Grummt, 2003; McStay & Grummt, 2008)
Summary
Previous reports have shown that adenovirus recruits nucleolar protein upstream-binding factor (UBF) into adenovirus DNA replication centres. During adenovirus replication this cellular protein is sequestered from the nucleolus into adenoviral DNA replication centres (Lawrence et al, 2006). We speculated that other DNA viruses might interact with this cellular protein and examined herpes simplex virus type 1 (HSV-1).
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