Abstract
The gene encoding diacylglycerol acyltransferase (DGAT1) is a functional and positional candidate gene for milk and intramuscular fat content. A bovine DGAT1 overexpression vector was constructed containing mouse MCK promoter and bovine DGAT1 cDNA. MCK-DGAT1 transgene in FVB mice was researched in present study. The transgene DGAT1 had a high level of expression in contrast to the endogenous DGAT1 in posterior tibial muscle of the transgenic mice, but a low expression level in the cardiac muscle. Compared with wild-type mice, triglyceride and DGAT1 content were approximately fourfold and 50% increased in posterior tibial muscle of the transgenic mice, respectively, while a little increase in cardiac muscle.
Highlights
Triacylglycerols (TG) are quantitatively the most important storage form of energy for eukaryotic cells
The RT gene expression analysis was performed on five transgenic mice and five WT mice, which showed that expression of the transgene was primarily restricted to skeletal muscle, and had a high level contrast to the endogenous Diacylglycerol acyltransferase 1 (DGAT1) in posterior tibial muscle of the transgenic mice (Figure 1A), but a very low expression level in the cardiac muscle (Figure 1B)
Increased TG and DGAT1 content in skeletal muscle of Muscle creatine kinase (MCK)-DGAT1 transgenic mice 10 – 12 weeks mice were used in this study
Summary
Triacylglycerols (TG) are quantitatively the most important storage form of energy for eukaryotic cells. Diacylglycerol acyltransferase 1 (DGAT1) encodes acyl CoA: diacylglycerol acyltransferase (DGAT) which catalyzes the terminal and only committed step in triacylglycerol synthesis [1]. DGAT1 is present in all cell types but is most highly expressed in tissues and organs where triglyceride synthesis is most active, including adipose tissue, liver, skeletal muscle and small intestine. DGAT deficiency alters triglyceride metabolism in mammary gland of mice [3]. Thaller et al [4] treated DGAT1 as a positional and functional candidate gene for intramuscular fat deposition in cattle. Myocellular overexpression of DGAT1 resulted in increased intramyocellular TG levels but decreased myocellular DAG and ceramide levels [5]
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