Abstract
ABSTRACTObjectives: To explore the role of SEPT7 in glioma cell invasion. Methods: SEPT7 was transfected into human glioma cell lines U251 and TJ899, the invasive abilities were evaluated by transwell assay, scratch assay, and 3-D/2-D Matrigel growth. The expression of MMP2/9, MT1-MMP, integrin αvβ3, and TIMP1/2 was detected by immunohistochemistry, immunofluorescence, and Western blot analyses. Distribution of α-tubulin was examined by laser scanning confocal analysis. Result: After SEPT7 trasfection, cell invasion was inhibited, expression of MMP2/9, MT1-MMP, and integrin αvβ3 was decreased, while TIMP1/2 was increased, and α-tubulin was redistributed. Conclusion: These results suggest that SEPT7 plays an important role in the glioma cell invasion.
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