Abstract
The expression pattern of pluripotency markers in adipose tissue-derived stem cells (ADSCs) is a subject of controversy. Moreover, there is no data about the signaling molecules that regulate these markers in ADSCs. In the present study, we studied the roles of leukemia inhibitory factor (LIF) and miR-302 in this regard. Freshly isolated mouse ADSCs expressed hematopoietic, mesenchymal, and pluripotency markers. One day after plating, ADSCs expressed OCT4 and Sox2 proteins. After three passages, the expression of hematopoietic and pluripotency markers decreased, while the expression of mesenchymal stem cell markers exhibited a striking rise. Both supplementation of culture media with LIF and transfection of the ADSCs with miR-302 family upregulated the expression levels of OCT4, Nanog, and Sox2 mRNAs. These findings showed that mouse adipose tissue contains a population of cells with molecular resemblance to embryonic stem cells, and LIF and miR-302 family positively affect the expression of pluripotency markers.
Highlights
White adipose tissue represents a rich source of stem cells with potential applications in basic and clinical research
Freshly isolated mouse Adipose tissue-derived stem cells (ADSCs) showed the expression of pluripotency markers at mRNA and protein levels
The expression of pluripotency markers was eliminated, while the expression of mesenchymal cellspecific markers showed a striking enhancement. These findings show that white adipose tissue is containing a population of pluripotent stem cells with molecular resemblance to embryonic stem (ES) cells
Summary
White adipose tissue represents a rich source of stem cells with potential applications in basic and clinical research. Adipose tissue-derived stem cells (ADSCs) can be harvested from patients by a simple and minimally invasive method. They can be cultured and rapidly propagated [1]. Peroni and colleagues [6] showed that bone marrow-derived mesenchymal stem cells (BM-MSCs) and ADSCs have a virtually identical transcriptional profile for stemness-related genes. Both cells express embryonic stem (ES) cell-specific genes, including OCT4, UTF1, and Nodal. This finding was against a previous report by Case and colleagues [7]. There is no data about the signaling molecules that regulate these markers in the ADSCs
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