Abstract
BackgroundOne of the hallmarks of cancer is an altered energy metabolism, and here, mitochondria play a central role. Previous studies have indicated that some mitochondrial ribosomal proteins change their expression patterns upon transformation.MethodIn this study, we have used the selection of recombinant antibody libraries displayed on the surface of filamentous bacteriophage as a proteomics discovery tool for the identification of breast cancer biomarkers. A small subpopulation of breast cells expressing both cytokeratin 19 and cytokeratin 14 was targeted using a novel selection procedure.ResultsWe identified the mitochondrial ribosomal protein s18a (Mrps18a) as a protein which is upregulated in breast cancer. However, Mrps18a was not homogeneously upregulated in all cancer cells, suggesting the existence of sub-populations within the tumor. The upregulation was not confined to cytokeratin 19 and cytokeratin 14 double positive cells.ConclusionThis study illustrates how phage display can be applied towards the discovery of proteins which exhibit changes in their expression patterns. We identified the mitochondrial protein Mrps18a as being upregulated in human breast cancer cells compared to normal breast cells.
Highlights
One of the hallmarks of cancer is an altered energy metabolism, and here, mitochondria play a central role
We identified the mitochondrial ribosomal protein s18a (Mrps18a) as a protein which is upregulated in breast cancer
We identified the mitochondrial protein Mrps18a as being upregulated in human breast cancer cells compared to normal breast cells
Summary
One of the hallmarks of cancer is an altered energy metabolism, and here, mitochondria play a central role. Sørensen et al BMC Cancer (2017) 17:19 These early studies revealed that members of the s18 protein family are localized on the surface of the small subunit (S28) of the mitochondrial ribosome, the function of these proteins is largely unknown. A study of overexpression of Mrps18b in human breast and renal cancer cell lines has shown that Mrps18b results in the appearance of multinucleated cells [10]. These observations indicate that Mrps family proteins, or at least Mrps18b, may be involved in neoplastic transformation
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