Up-regulation of lipogenic enzyme genes expression in inguinal white adipose tissue of female rats by progesterone

Abstract

Contradictory results have been published regarding the influence of progesterone on lipids metabolism in adipose tissue. The aim of the present work was to elucidate whether progesterone administration in the setting of an experimental model influences lipogenic enzyme genes expression, body and adipose tissue mass. The results presented here indicate that the elevated blood progesterone concentration was associated with significant increase in lipogenic enzyme genes expression in inguinal adipose tissue of females. The rise in the expression of lipogenic enzyme genes was associated with an increase in sterol regulatory element binding transcription factor 1 (Srebf1) and S14 genes expression. Mifepristone, a specific antagonist of progesterone receptor, abolished progesterone's effect on body mass, inguinal fat mass, and lipogenic enzyme genes expression in inguinal adipose tissue. No significant changes were found in the expression of lipogenic enzyme genes, Srebf1 and S14 genes in perirenal white adipose tissue of females. The elevated blood progesterone concentration was associated with the increase in body and inguinal white adipose tissue mass of females. In males, elevated blood progesterone concentration had no effect on the lipogenic enzyme genes expression and on body and fat mass.In conclusion, we demonstrate that a chronic increase in serum progesterone concentration in females was associated with up-regulation of lipogenic enzyme genes expression in inguinal adipose tissue. Up-regulation of Srebf1 and S14 genes expression following progesterone administration suggests that products of these genes might be involved in the regulation of lipogenic enzyme genes expression by progesterone. The stimulatory effect of progesterone on lipogenic enzyme genes expression in inguinal adipose tissue seems to be specific as it was reversed by specific antagonist of progesterone receptor.