Abstract
Lung cancer (LC) is a malignant tumor with the highest incidence and mortality rates worldwide. Linc00284, a long non-coding RNA, is a newly discovered regulator of LC. This study aimed to explore the role of Linc00284 in LC progression. Gene expression levels were detected by RT-qPCR and/or western blot analysis. Cell migratory and invasive capabilities were measured by wound healing and transwell assays. Subcutaneous xenograft models were constructed to examine tumor growth of LC cells. Data showed that Linc00284 was significantly upregulated in LC tissues compared to adjacent normal lung tissues and predicted poor prognosis in patients with LC. In vitro, Linc00284 was highly expressed in LC cells and was mainly localized in the cytoplasm. Mechanistically, Linc00284 directly bound to miR-205-3p, leading to the upregulation of c-Met expression. A significant negative correlation was observed between Linc00284 and miR-205-3p expression levels, and the Linc00284 level was positively correlated with the c-Met expression. Linc00284/miR-205-3p/c-Met regulatory axis promotes LC cell proliferation, migration, and invasion. Furthermore, the in vivo results indicated that Linc00284 knockdown markedly suppressed tumor growth. Taken together, these data suggest that Linc00284 facilitates LC progression by targeting the miR-205-3p/c-Met axis, which may be a potential target for LC treatment.
Highlights
Lung cancer (LC) is the leading cause of cancer-associated mortality worldwide (Siegel et al, 2020)
Following the extraction of total RNA and performing RT-qPCR, it was found that Linc00284 expression was significantly upregulated in LC tissues compared with adjacent normal samples (Figure 1A)
Linc00284 was highly expressed in tumor tissues from lung cancer patients with metastasis/recurrence compared to those patients without metastasis/recurrence (Figures 1C,D)
Summary
Lung cancer (LC) is the leading cause of cancer-associated mortality worldwide (Siegel et al, 2020). Linc00284 Promotes Lung Cancer Progression processes (Yang et al, 2014; Gao et al, 2018; Lei et al, 2019). LincRNAs belong to long non-coding RNAs (lncRNAs; Deniz and Erman, 2017; Ransohoff et al, 2018). LincRNAs are significantly associated with the development and progression of various cancer types (Chen et al, 2017; Ma et al, 2018). LincRNAs modulate oncogene expressions, promoting the malignant phenotypes of tumors, including LC (Lu et al, 2017; Peng et al, 2018; Wang et al, 2019), gastric cancer (Chen et al, 2018), and colon cancer (Chang et al, 2018)
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