Abstract
Gastric cancer is the fifth most common cancer worldwide with a poor survival rate. Therefore, it is important to identify predictive and prognostic biomarkers of gastric cancer. Laminin subunit beta 1 (LAMB1) is involved in attachment, migration, and organization during development, and its elevated expression has been associated with several cancers. However, the role and mechanism of LAMB1 in gastric cancer remains unknown. Here, we determined that LAMB1 is upregulated in gastric cancer tissues and contributes to cell growth and motility. Using a public database, we showed that LAMB1 expression was significantly upregulated in gastric cancer compared to normal tissues. LAMB1 was also found to be associated with poor prognosis in patients with gastric cancer. Overexpression of LAMB1 elevated cell proliferation, invasion, and migration; however, knockdown of LAMB1 decreased these effects in gastric cancer cells. U0126, an extracellular signal-regulated kinase (ERK) inhibitor, regulated the expression of LAMB1 in gastric cancer cells. Additionally, we showed that c-Jun directly binds to the LAMB1 promoter as a transcription factor and regulates its gene expression via the ERK pathway in gastric cancer cells. Therefore, our study indicates that LAMB1 promotes cell growth and motility via the ERK/c-Jun axis and is a potential biomarker and therapeutic target of gastric cancer.
Highlights
Gastric cancer is the most common contributor to the global burden of malignant tumors and responsible for a high mortality rate worldwide [1,2]
To identify the genes that are overexpressed in gastric cancer, we used the public database of four gene expression omnibus (GEO) datasets (GSE2685, GSE13861, GSE33651, and GSE63089) to analyze the differentially expressed genes (DEGs)
Overexpression of Laminin subunit beta 1 (LAMB1) sion survival of gastric cancer patients. These results suggest was overexpression associated with poor overallmight survival first progression post-progression that of LAMB1 be a(OS), potential indicator for(FP), poorand prognosis in gastric survival (PPS) of gastric cancer patients (Figure 2C and Figure S2)
Summary
Gastric cancer is the most common contributor to the global burden of malignant tumors and responsible for a high mortality rate worldwide [1,2]. Despite the advancements in various cancer therapies, such as radiation therapy, immunotherapy, chemotherapy and surgery, the five-year relative cancer survival rate remains poor with approximately. There is an urgent need to develop the diagnosis and new therapies for gastric cancer, for which it is important to identify effective prognostic markers. LAMB plays a role in initiating cell assembly by combining with LAMC. Cell assembly plays an important role in cancerous cell invasion and metastasis [5]. It has been reported that LAMB1 is highly expressed in several invasive cancers [6]
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