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Abstract
The abnormal increase in proliferation rate of human periodontal ligament stem cells (PDLSCs) is considered to be involved in the pathogenesis of periodontitis, a disease in which the IL-10-mediated anti-inflammatory pathway plays a critical role. This study aimed to investigate the involvement of microRNA-466l in periodontitis and to explore the possible interaction between IL-10 and microRNA-466l. PDLSCs were obtained from periodontitis-affected teeth and healthy control teeth. The expression of microRNA-466l and IL-10 mRNA was measured in PDLSCs using RT-qPCR. The proliferation ability of PDLSCs was analyzed using CCK-8 assays. Overexpression of microRNA-466l in a PDLSC cell line was established using two different types of PDLSCs, and the effect of microRNA-466l overexpression on IL-10 expression and cell proliferation were detected by western blot and CCK-8 assays, respectively. We found that expression levels of microRNA-466l and IL-10 mRNA were significantly lower (P < 0.05) in PDLSCs derived from periodontitis-affected teeth compared to those derived from healthy teeth. However, the cell proliferation ability was significantly higher in the PDLSCs derived from periodontitis-affected teeth. Meanwhile microRNA-466l overexpression decreased cell proliferation rates of both types of PDLSCs and upregulated IL-10 expression. Together, these data suggest that microRNA-466l can upregulate IL-10 and reduce the proliferation rate of PDLSCs.
Highlights
Periodontitis is a local lesion in mouth, and a dysbiotic inflammatory disease that affects systemic health 1
Decreased expression of microRNA-466l and IL-10 mRNA in periodontal ligament stem cells (PDLSCs) derived from periodontitis-affected teeth
We detected the expression of microRNA-466l and IL-10 mRNA in PDLSCs derived from periodontitis-affected teeth and healthy teeth using RT-qPCR
Summary
Periodontitis is a local lesion in mouth, and a dysbiotic inflammatory disease that affects systemic health 1. In the United States, periodontitis affects more than 50% of adults over the age of 30 2. In China, the poor overall oral health conditions make periodontitis a substantial burden on public health 3. It has been reported that the occurrence of periodontitis is correlated with multiple factors, including diabetes, being male, and having a low education level 4. Treatment of periodontitis is most frequently accomplished using systemic antibiotics. The misuse of antibiotics, such as overdoses and improper drug selections, can lead to other serious and unfavorable health conditions, including drug resistance 5
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