Abstract
The purpose of this study was to examine the regulation of A 2A adenosine receptor (A 2A AR) gene expression induced by proinflammatory cytokines in PC12 cells. The A 2A AR mRNA levels were substantially increased following 3–48 hr PC12 cell treatment with interleukin 1 beta (500 unit/mL) or tumor necrosis factor alpha (1000 unit/mL), as revealed by RT-PCR analysis. In parallel, cell cytokine treatment induced an up-regulation of A 2A receptor protein. Equilibrium radioligand binding studies on treated-cells showed a significant increase in maximum density of [ 3 H ] 2-(carboxyethylphenylethylamino) adenosine-5′-carboxamide binding sites, with no significant changes in the affinity constant value. The increase in A 2A receptor density was also demonstrated by Western blot analysis. Interleukin 1 beta and tumor necrosis factor alpha effects on A 2A AR mRNA and protein levels were detectable after 3 hr cytokine treatment and reached a maximum within 24 and 48 hr, respectively. These results demonstrated the existence of heterologous regulation of A 2A ARs by proinflammatory cytokines. The biological significance of this regulation might be associated with modulating cellular activity in response to tissue damage associated with inflammatory mediator production.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
