Abstract
BackgroundLong noncoding RNAs (lncRNAs) play widespread roles in gene regulation and cellular processes. However, the functional roles of lncRNAs in colorectal cancer (CRC) are not yet well elucidated. The aim of the present study was to measure the levels of lncRNA 91H expression in CRC and evaluate its clinical significance and biological roles in the development and progression of CRC.Methods91H expression and copy number variation (CNV) were measured in 72 CRC tumor tissues and adjacent normal tissues by real-time PCR. The biological roles of 91H were evaluated by MTT, scratch wound assay, migration and invasion assays, and flow cytometry.Results91H was significantly overexpressed in cancerous tissue and CRC cell lines compared with adjacent normal tissue and a normal human intestinal epithelial cell line. Moreover, 91H overexpression was closely associated with distant metastasis and poor prognosis in patients with CRC, except for CNV of 91H. Multivariate analysis indicated that 91H expression was an independent prognostic indicator, as well as distant metastasis. Our in vitro data indicated that knockdown of 91H inhibited the proliferation, migration, and invasiveness of CRC cells.Conclusions91H played an important role in the molecular etiology of CRC and might be regarded as a novel prognosis indicator in patients with CRC.
Highlights
Colorectal cancer (CRC) is a common cause of cancer death in the world due to late tumor presentation and rapid progression, with about 14.1 million new cancer cases and 8.2 million cancer deaths in 2012 worldwide [1]
Despite less well understanding of Long noncoding RNAs (lncRNAs) compared with microRNAs [3,4,5], accumulating evidences indicate that lncRNAs-mediated biology could be involved in regulation of diverse cellular processes, such as cell growth and apoptosis, stem cell pluripotency and development, meiotic entry and telomere length [6,7,8,9,10,11]
The 91H expression levels were determined by quantitative real-time PCR (qRT-PCR) for 72 cancerous and adjacent noncancerous tissues from colorectal cancer (CRC) patients. 91H levels in cancerous tissues were obviously higher than those in the noncancerous tissues (P,0.001; Figure 1)
Summary
Colorectal cancer (CRC) is a common cause of cancer death in the world due to late tumor presentation and rapid progression, with about 14.1 million new cancer cases and 8.2 million cancer deaths in 2012 worldwide [1]. Despite substantial progress achieved in diagnosis and treatment for CRC in recent years, the overall 5-year survival rate remains unsatisfactory due to metastasis leading to poor outcomes [3]. Recent studies have revealed that lncRNAs, .200 nucleotides in length, play a critical role in cancer development and progression. High expression of PVT-1 expression in cancerous tissues was regarded as an independent risk factor for overall survival of CRC patients [12], and HOTAIR, functioning as an PLOS ONE | www.plosone.org. The functional roles of lncRNAs in colorectal cancer (CRC) are not yet well elucidated. The aim of the present study was to measure the levels of lncRNA 91H expression in CRC and evaluate its clinical significance and biological roles in the development and progression of CRC
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