Abstract
Recent reports have shown that nuclear enriched abundant transcript 1 (NEAT1), a long non- coding RNA (lncRNA), contributes to the precise control of gene expression and is related to several human malignancies. However, limited data are available on the expression and function of NEAT1 in lung cancer. The major objective of the current study was to profile the expression and clinicopathological significance of NEAT1 in non-small cell lung cancers (NSCLCs). NEAT1 expression in 125 NSCLC cases and paired adjacent non-cancer tissues was assessed by real-time quantitative reverse transcription-PCR (qRT-PCR). Relationships between NEAT1 and clinicopathological factors were also investigated. The relative level of NEAT1 was 6.98±3.74 in NSCLC tissues, significantly elevated as compared to that of the adjacent non-cancer lung tissues (4.83±2.98, p<0.001). The area under curve (AUC) of high expression of NEAT1 to diagnose NSCLC was 0.684 (95% CI: 0.619~0.750, p<0.001). NEAT1 expression was positively correlated with patient age (r=-2.007, p=0.047), lymphatic metastasis (r=-2.731, p=0.007), vascular invasion (r=-3.617, p=0.001) and clinical TNM stage (r=-4.134, p<0.001). This study indicates that NEAT1 might be associated with oncogenesis and progression in NSCLC, and suggests application in molecular targeted therapy.
Highlights
According to recent estimates, lung cancer remains as the leading cause of cancer-related deaths all over the world (Siegel et al, 2014), with an estimated 1.4 million deaths per year (Barrow and Michels, 2014)
NEAT1expression in non-small cell lung cancers (NSCLCs) tissues (AUC) of nuclear enriched abundant transcript 1 (NEAT1) was 0.684 (95%CI: 0.619~0.750, The OD260/OD280 ratio of the total mRNA isolated p
No study has been reported to investigate the distinction of NEAT1 expression between NSCLC and normal lung tissues
Summary
Lung cancer remains as the leading cause of cancer-related deaths all over the world (Siegel et al, 2014), with an estimated 1.4 million deaths per year (Barrow and Michels, 2014). Nuclear enriched abundant transcript 1(NEAT1) is a nuclear-restricted lncRNA, which owns two isoforms: 3.7 kb NEAT1_1 and 23 kb NEAT1_2(Naganuma and Hirose, 2013) This lncRNA has been recently revealed to be an architectural component of a subnuclear structure called the paraspeckle, which is suggested to be involved in regulating gene expression by retaining mRNAs for editing in the nucleus (Chen and Carmichael, 2009). Anomalous NEAT1 expression has been reported in human malignancies, including of leukemia and ovarian carcinoma (Kim et al, 2010; Zeng et al, 2014). Recent reports have shown that nuclear enriched abundant transcript 1 (NEAT1), a long noncoding RNA (lncRNA), contributes to the precise control of gene expression and is related to several human malignancies. The major objective of the current study was to profile the expression and clinicopathological significance of NEAT1 in non-small cell lung cancers (NSCLCs). Conclusions: This study indicates that NEAT1 might be associated with oncogenesis and progression in NSCLC, and suggests application in molecular targeted therapy
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
