Upregulating the Expression of F-CAR-T Cells by Increasing theAmount of Tcm Cells in F-CAR-T Cells

Abstract

CAR-T cells are most known for protecting one’s immune system by attacking cancer cells. Usually, it is infused intothe patient’s body to provide treatment. When comparing F-CAR-T cells with C-CAR-T cells, F-CAR-T cells typicallyhave more Tscm cells. Thus, this study investigates the effect of upregulating Tscm cells in F-CAR-T cells using FACSfor CD45RO- and CD62L+. The experiments will use the expression of Tim3, LAG3, and PD1 and measure if F-CAR-Tcells can kill CD19-expressing RAJI cells by FACS for CD19 and Annexin V/PI through various durations and withvarious numbers of injected CarT. Then Tscm cells are measured by FACS for CD45RO- and CD62L+. Moreover,two special diets will be used as an experimental control to regulate the increase and decrease of Tscm cells. There aretwo most possible results: (1) Both the Tscm receptor and the diet will have a positive effect in terms of upregulatingTscm cells in F-CAR-T cells; (2) Both the mouse peritoneal macrophage and the diet will inhibit the growth of Tscmcells. The result of our study will provide important information for the future clinical trial of Tscm cell application andimprove CAR-T cells’ persistence. Future studies should focus on shortening the CAR-T cells manufacturing processesas well as exploring more applicable usages of Tscm cells in detail