Abstract
Prostate cancer (PCa) is the second-leading cause of cancer-related death among men. microRNAs have been identified as having potential roles in tumorigenesis. An oncomir, miR-21, is commonly highly upregulated in many cancers, including PCa, and showed correlation with the Wnt-signaling axis to increase invasion. Wnt-11 is a developmentally regulated gene and has been found to be upregulated in PCa, but its mechanism is unknown. The present study aimed to investigate the roles of miR-21 and Wnt-11 in PCa in vivo and in vitro. First, different Gleason score PCa tissue samples were used; both miR-21 and Wnt-11 expressions correlate with high Gleason scores in PCa patient tissues. This data then was confirmed with formalin-fixed paraffin cell blocks using PCa cell lines LNCaP and PC3. Cell survival and colony formation studies proved that miR-21 involves in cells’ behaviors, as well as the epithelial-mesenchymal transition. Consistent with the previous data, silencing miR-21 led to significant inhibition of cellular invasiveness. Overall, these results suggest that miR-21 plays a significant role related to Wnt-11 in the pathophysiology of PCa.
Highlights
Prostate cancer (PCa) is the development of carcinoma within the prostate gland of the male reproductive system
We reveal that silencing miR-21 expression results in significant inhibition of cellular invasiveness
In this study, we showed that miR-21 plays an important role during cell migration and invasion
Summary
Prostate cancer (PCa) is the development of carcinoma within the prostate gland of the male reproductive system. It is a heterogeneous disease and may present itself as an indolent or aggressive form [1]. There is no specific form of screening for PCa, and health practitioners may use a combination of several tests in order to diagnose a patient. Tumors with lower GS indicate cells that are similar to normal cells and are likely to be less aggressive. Those assigned with higher scores are indicative of tumors with poorly
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
