Up-Regulated Genes in Cell Cycle Pathway of ccRCC Discovered by High-Throughput Sequencing Data Mining

Abstract

To sequence the transcriptome of renal clear cell carcinoma with RNA-seq technology, so as to verify the cell cycle genes, gene combinations, or pathways known to be related to clear cell renal carcinoma (ccRCC) from the transcriptional level. To search for potential gene combinations and pathways that may affect the proliferation, differentiation, and tumor progression of ccRCC. Methods: Samples of renal clear cell carcinoma and normal tissue around were extracted, and then ccRCC and normal renal tissue cDNA libraries were constructed, and SOLID™ 3.0 sequencing system was used for mRNA sequencing. Results: After sequencing and transcriptome related to the cell cycle were obtained, combined with gene expression level data software, the known genes related, combination of genes, or access the expression level of verification, it was found that most of the genes in the ccRCC cell cycle pathway are up-regulated, which leads to the disorder of cell cycle, accelerates cell division, destroys the cell cycle monitoring mechanism, and eventually leads to cancer. Conclusion: The results will provide some basic information for the diagnosis and treatment of ccRCC.