Abstract
Xiaoyao Powder (XYP), a common Chinese medicine, comprises eight traditional Chinese herbs and has been widely used clinically to treat liver damage and mental disorders. An ultra-performance liquid chromatography–tandem mass spectrometry method was developed to investigate the pharmacokinetics of 14 compounds (albiflorin, paeoniflorin, ferulic acid, senkyunolide I, quercetin, isoliquiritigenin, atractylenolide III, ligustilide, atractylenolide II, liquiritin, liquiritigenin, saikosaponin c, glycyrrhizic acid, and saikosaponin a) in XYP. Naringenin was used as the internal standard. The compounds were separated using an ACQUITY UPLCTM BEH C18 column (1.7 μm, 50 × 2.1 mm) with a mobile phase consisting of acetonitrile and 0.1% formic acid in water at a flow rate of 0.3 mL/min. Detection was performed on a triple-quadrupole tandem mass spectrometer using multiple reaction monitoring and an electrospray ionization source in both positive and negative ionization modes. All calibration curves exhibited good linearity (r2 > 0.9974) over the measured ranges. The intra- and inter-day precisions were within 12%, and the accuracy ranged from 89.93% to 106.64%. Extraction recovery and matrix effect results were satisfactory. The method was successfully applied in a pharmacokinetic study of the 14 compounds in rat plasma after the oral administration of XYP.
Highlights
Xiaoyao Powder (XYP), a traditional Chinese medicine formula, was first described in the Taiping Huimin Heji Jufang during the Song Dynasty of China (960–1127 AD)
The components of XYP were identified by ultra-performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry (UPLC-ESI-MS)
Under the established optimal chromatographic conditions, no significant interfering peaks were observed at the analyte elution times, and no interference occurred between the internal standard (IS) and the 14 analytes
Summary
Xiaoyao Powder (XYP), a traditional Chinese medicine formula, was first described in the Taiping Huimin Heji Jufang during the Song Dynasty of China (960–1127 AD). This formula has been widely used to treat liver damage and as an antidepressant for more than 2000 years [1,2,3,4]. The components of XYP were identified by ultra-performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry (UPLC-ESI-MS). Gas chromatography-mass spectrometry (GC-MS), yielding a comprehensive list of compounds in XYP [6,7]. There are no reports on which of these XYP compounds are absorbed, nor which may have a key role in the pharmacokinetics of XYP
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