Abstract
The quality control of Polygala tenuifolia Wild. is a major challenge in its clinical application. In this paper, a new strategy for the quality evaluation of P. tenuifolia extracts was verified through reverse-phase ultra-performance liquid chromatography (UPLC). The quantitative analysis of multi-components by a single marker (QAMS) was conducted with 3,6′-disinapoyl sucrose as an internal reference substance. Eight components (i.e., sibiricose A5, sibiricose A6, glomeratose A, tenuifoliside A, tenuifoliside B, tenuifoliside C, sibiricaxanthone B, and polygalaxanthone III) were determined based on the relative correction factors. The concentrations of these components were also determined by applying a conventional external standard method. The cosine value confirmed the consistency of the two methods (cosine ratio value >0.999920). Hierarchical cluster analysis, radar plots, and discriminant analysis were performed to classify 23 batches of P. tenuifolia extracts from Shanxi, Hebei, and Shaanxi in China. Results revealed that QAMS combined with radar plots and multivariate data analysis could accurately measure and clearly distinguish the different quality samples of P. tenuifolia. Hence, QAMS is a feasible and promising method for the quality control of P. tenuifolia.
Highlights
Traditional Chinese medicine (TCM) has gained increasing attention worldwide; in this regard, ensuring the curative effects of TCM has become an important issue [1,2]
QAMS was developed for the simultaneous quantitative analysis of nine components in 23 batches of P. tenuifolia by ultra-performance liquid chromatography (UPLC)
The relative calibration factors (RCFs) between disinapoyl sucrose (DISS) and the other eight components were investigated with QAMS calculations under different instruments, columns, column temperatures, and flow rates of the mobile phase
Summary
Traditional Chinese medicine (TCM) has gained increasing attention worldwide; in this regard, ensuring the curative effects of TCM has become an important issue [1,2]. The quality control of TCM has depended on only one single indicator, which cannot reflect its pleiotropy and integrity. The simultaneous determination of multi-components (SDMC) is used to characterize herbal or botanical products. Chemical fingerprint analysis is used to evaluate the quality of herbal drugs, identify their authenticity, and differentiate their origins [3]. As an important part of fingerprint analysis, reverse-phase ultra-performance liquid chromatography (UPLC) exhibits significant higher peak resolution, sensitivity, and speed of analysis than high-performance liquid chromatography (HPLC) [4,5]. UPLC utilizes high linear velocities, with columns packed with particles that are less than
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