UPLC-MS/MS and Dushman reaction based spectrophotometric method for determination of Ceftazidime, an antibiotic, in medicinal formulation

Masoom Raza Siddiqui,Fohad Mabood Husain,Saikh Mohammad Wabaidur,Afnan Ali Hussain Hakami,Zeid Abdullah Alothman,Moonis Ali Khan

doi:10.1590/fst.07020

Masoom Raza Siddiqui, Fohad Mabood Husain + Show 4 more

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https://doi.org/10.1590/fst.07020

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Abstract

ABSTRACT: Two analytical methods were developed, one utilizing sensitive UPLC-MS/MS method while other being the spectrophotometric method exploiting the Dushman reaction. Both methods were validated based on ICH guidelines and were successfully applied to quantitate the drug in marketed formulation. UPLC-MS/MS method responded linearly in the range of 1.6 µgmL-1-6.4 µgmL-1 while spectrophotometric procedure followed the Beer’s law in the concentration range of 30-100 µgmL-1. UPLC-MS/MS has been found to have a limit of quantitation of 0.97 µgmL-1 while spectrophotometric method was found to quantitate 14.00 µgmL-1 correctly with high precision. Both the methods showed an excellent recovery of 99.36%-100.91% (% RSD 1.05-1.73) and 98.64%-99.73% (%RSD 0.67-1.68) for UPLC-MS/MS and spectrophotometric, respectively.

Highlights

Ceftazidime, a third-generation cephalosporin with greater activity against Gram-negative bacteria is chemically recognized as (6R, 7R, Z)-7-(2-(2-aminothiazol-4-yl)-2-(2-carboxypropan2-yloxyimino) acetamido)-8-oxo-3-(pyridinium-1-ylmethyl)5-thia-1-aza-bicyclo [4.2.0] oct-2-ene-2-carboxylate.Like other third generation cephalosporins, it is stable enough to resist inactivation by hydrolysis via b-lactamases produced by the gram +ve and gram –ve bacteria
Cheshire, UK manufactured ethanol was used for the UPLC-MS/MS studies, whereas, Milli Q purified water was used throughout the experiment
The spectrophotometric measurements were carried out using Thermo manufactured, Evolution 300 UV-visible spectrophotometer; quartz cells were used as sample holder and the reaction were measured at 25 °C, and the yellow colored complex of ceftazidime-iodide-iodate was measured at 352 nm

Summary

Introduction

Ceftazidime, a third-generation cephalosporin with greater activity against Gram-negative bacteria is chemically recognized as (6R, 7R, Z)-7-(2-(2-aminothiazol-4-yl)-2-(2-carboxypropan2-yloxyimino) acetamido)-8-oxo-3-(pyridinium-1-ylmethyl)5-thia-1-aza-bicyclo [4.2.0] oct-2-ene-2-carboxylate.Like other third generation cephalosporins, it is stable enough to resist inactivation by hydrolysis via b-lactamases produced by the gram +ve and gram –ve bacteria. Ceftazidime, a third-generation cephalosporin with greater activity against Gram-negative bacteria is chemically recognized as (6R, 7R, Z)-7-(2-(2-aminothiazol-4-yl)-2-(2-carboxypropan2-yloxyimino) acetamido)-8-oxo-3-(pyridinium-1-ylmethyl)5-thia-1-aza-bicyclo [4.2.0] oct-2-ene-2-carboxylate. The uniqueness of ceftazidime in its class of cephalosporin is its activity against the Pseudomonas species (Otani et al, 2018). Cystic fibrosis and meningitis are the other conditions where ceftazidime treatment is recommended (Gozzard et al, 1982). Another import use of the drug includes its all-important usage in diabetic foot syndrome. World health organization has classified ceftazidime in the list of essential medicines. The list contains important drugs which is needed for basic health care. As reported the human body practically cannot metabolize ceftazidime, 90-96% of ceftazidime is excreted in unaltered form (Tůma et al, 2016)

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