Abstract
In the current study, the active flavonoids from Apocynum venetum and Apocynum hendersonii leaf were efficiently characterized using UPLC-ESI-MS/MS, and yielding the highest content of 15.35 mg/g (A. venetum) and 13.28 mg/g (A. hendersonii) respectively. The antioxidant assay in vitro showed that the isolated flavonoid ingredient groups exhibited free radical scavenging activities to DPPH, ABTS and linoleic acid. The antimicrobial assay revealed the isolated flavonoid ingredient from both A. venetum and A. hendorsonii have exerted anti-MRSA and anti-P. aeruginosa effect through disrupting cell integrity and declining ATP. In vivo assay demonstrated that these flavonoid ingredients effectively accelerated MRSA-infected and P. aeruginosa-infected Balb/c mice wound healing. In summary, these results showed that the characterized flavonoid ingredients exhibited great potential as natural antioxidant and antimicrobial agents, and shed light into future potential applications of Apocynum spp.
Highlights
IntroductionPublisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations
The components of A. venetum and A. hendersonii leaf samples were extracted by high-performance liquid chromatography (HPLC), and the presence of tentative flavonoids were identified by comparing the retention time and the corresponding peak of UV spectra with those of standards
The results indicated that the isolated flavonoids from A. hendersonii leaf showed the strongest inhibitory effect and antibacterial activity, which was confirmed by the lowest optical density compared to that of A. venetum
Summary
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations. Apocynum spp., commonly known as “Luobuma” in Chinese is a perennial herbaceous widely distributed in saline–alkali land, riverbanks, sandy soils and fluvial plains [1]. As a genus of the plant family Apocynaceae [2,3], it is mainly divided into Apocynum venetum. L. and Apocynum hendersonii Hook.f [4], two species with important medicinal perennial rhizomatous plants with ecological and economic value [2,5]. The utilization of Apocynum spp. was mainly focused on the textile application of phloem fibers [6], while chemical degumming technology for Apocynum spp. fiber preparation often caused environment pollution due to the use of chunk of chemicals [5]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
