Abstract
Objective: To establish the UPLC-ELSD fingerprint of Dipsaci Radix and provide reference for the quality control of Dipsaci Radix. Methods: Agilent ZORBAX RRHD Eclipse Plus C18 (2.1mm×100mm, 1.8μm) column was used, the mobile phase was composed of acetonitrile-water, gradient elution, the flow rate was 0.2 mL/min, atomization temperature of evaporative light scatter detector was 60°C, the column temperature was 30°C, nitrogen carrier gas flow rate was 1.5 L/min. Similarity evaluation was used to adjust 17 batches of Dipsaci asper. Results: The UPLC-ELSD fingerprint of Dipsaci Radix Aspergillus was established. Seven common peaks were identified. The similarity of 17 batches of Dipsaci Radix. Aspergillus was between 0.659-1.000, which indicated that there were great differences among batches. Conclusion: The method is simple, efficient, rapid and reliable, it can provide reference for the quality evaluation of Dipsaci Radix.
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