Abstract
The growth and the intracellular biopolymer Poly-β Hydroxybutyrate (PHB) storage kinetics of Alcaligenes eutrophus were studied in a batch cultivation process. N C ratio was found to both limit and inhibit the growth of the culture. The effect of initial N C on μ max was studied in the range 0.03–0.4. Maximum μ max was observed at N C equal to 0.09. A structured mathematical model was proposed. The model parameters were evaluated using the actual batch fermentation data. The model was used to simulate nutrient feeding profiles for enhanced PHB production in a fed-batch fermentation.
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