Updated Gene Map of Tobacco Chloroplast DNA

Abstract

The choroplast DNA from tobacco (Nicotiana tabacum) has often served as areference for plastid genomes. It is now analyzed extensively due to advancesin transplastome techniques and in vitro technology. The complete nucleotidesequence and gene map was published in 1986 (Shinozaki et al., 1986a,b).Since then, 24 new genes have been identified and sequencing errors havebeen found. Genes found after 1986 include: one small RNA gene (sprA)and 23 protein-coding genes(psaC, psaI, psaJ, psbI, psbJ, psbK, psbL, psbM,psbN, psbT, petG, petL, ndhG, ndhH, ndhI, ndhJ, ndhK, rp132, rp136, rpoC2,matK, accD and clpP). Figure 1 shows the updated gene map which includes105 different genes and 9 ycfs.Some of the sequence errors were found by our group (e.g. Shimada etal., 1990), and the remaining errors were reported and suggested by othergroups (e.g. Olmstead et al., 1993). We have examined these errors by re-sequencing. All corrections (Table 1) were made to the original sequenceand the numbering system was changed accordingly. The updated gene list(Table 2) and sequence have been deposited with the EMBL Nucleotide Se-quence Database under the accession number Z00044. A printed sequence inwhich genes/ycfs/orfs (see Figure 1) are boxed is available upon request toM. Sugiura.