Abstract
Reticulocyte counts are expressed either as a proportion, as has been the practice for microscopic reticulocyte counts, or, preferably, in absolute terms, which are less ambiguous for clinical interpretation. The precision of reticulocyte counting has improved remarkably since the introduction of hematology analyzers and flow cytometers. In addition to reticulocyte counting, some instruments can be used to estimate the immature reticulocyte fraction (IRF). The IRF is useful in determining bone marrow function and response to treatment. Commercially prepared quality control materials are available for both instrumental and microscopic methods, and are used on a schedule similar to that for hematology controls. Control limits for the several reticulocyte counters are based on multiple analyses with the particular instrument. The assignment of control means and limits for the microscopic method is based on the new methylene blue method; however, certain common errors must be averted to obtain accurate reticulocyte counts on a continuing basis.
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