Abstract
Bone marrow transplantation (BMT) is a powerful strategy for the treatment of leukemia, aplastic anemia, congenital immunodeficiency and autoimmune diseases. In humans, bone marrow cells (BMCs) have usually been collected by multiple bone marrow aspirations from the iliac crest. We have established a new “perfusion” method for collecting BMCs with minimal contamination with the peripheral blood using the long bones of cynomolgus monkeys. This method has proven to be a simple and safe method for harvesting BMCs and reduces the risk of acute graft versus host disease in allogeneic BMT. Intra-bone marrow-BMT (IBM-BMT) provides distinct advantages because it recruits donor-derived hematopoietic stem cells and mesenchymal stem cells. IBM-BMT has been shown to currently be the best strategy for allogeneic BMT. Here we review the perfusion method (for harvesting BMCs) and IBM-BMT (for their transplantation) and show that this combination will become a powerful new clinical strategy for allogeneic BMT.
Highlights
Bone marrow transplantation (BMT) is a powerful strategy for the treatment of hematologic disorders, including leukemia and autoimmune diseases [1,2,3]
bone marrow cells (BMCs) harvested from the ilium of aged donors by the perfusion method can be used for BMT across major histocompatibility complex (MHC) barriers and for organ transplantation if the donors are brain dead
We previously showed that KK-Ay mice, a type 2 DM model reconstituted with KK-Ay bone marrow cells, showed glycosuria, hyperinsulinemia and hyperlipidemia
Summary
Bone marrow transplantation (BMT) is a powerful strategy for the treatment of hematologic disorders, including leukemia and autoimmune diseases [1,2,3]. Transplantation; BM: Bone Marrow; BMCs: Bone Marrow Cells; GvHD: Graft Versus-host Disease; HSCs: Hematopoietic Stem Cells; IBM-BMT: Intra-bone Marrow-BMT; IV-BMT: Intravenously Injected-BMT; MHC: Major Histocompatibility Complex; MSCs: Mesenchymal Stem Cells; PM: Perfusion Method Gene therapy and organ transplantation have been performed using bone marrow cells (BMCs) [4,5,6,7,8].
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
