Abstract
Purpose: Myocardial infarction (MI), known as a multifactorial disease, remains the predominant cause of mortality and sudden deaths annually. The current study aimed to measure the expression of microRNA-1 and microRNA-221-3p in MI patients. Methods: In the current study, 100 healthy controls (with no history of heart disease) and 200 patients with MI were selected. Patients were divided into two groups based on angiography results: normal (no significant artery stenosis) and primary percutaneous coronary intervention (primary PCI, significant artery stenosis). The levels of microRNA-1 and microRNA-221-3p were quantified using real-time quantitative polymerase chain reaction. The correlation between levels of microRNAs and the common cardiac markers were analyzed statistically. Results: In comparison to fold change, microRNA-1 elevations were 8.5-fold in normal patients and 60-fold in patients with primary PCI; while microRNA-221-3p levels were 210- fold higher in primary PCI and 31.31-fold higher in normal cases compared with the healthy controls. Receiver operating characteristic analysis showed that the area under the curve (AUC) for circulating microRNA-1 and microRNA-221 were 0.903 and 0.958 in normal patients and 0.927 and 0.985 in primary PCI patients (p < 0.0001), respectively. Pearson correlation (ρ) analysis showed that circulation of microRNA-1 correlated with serum levels of cardiac troponin I (CTnI) (ρ =0.24), creatinine (ρ =0.34), creatinine kinase-myocardial band (CK-MB) (ρ =0.31), and microRNA-221-3p was significantly correlated with serum levels of CTnI (ρ =0.6), creatinine (ρ =0.41), and CK-MB (ρ =0.37), (P < 0.0001). Conclusion: The study underscored the potential of microRNA-1 and microRNA-221-3p as informative biomarkers and positively correlated with artery stenosis in MI.
Highlights
In comparison to fold change, microRNA-1 elevations were 8.5-fold in c normal patients and 60-fold in patients with primary percutaneous coronary intervention (PCI); while microRNA-221c3p levels were 210-fold higher in primary PCI and 31.31-fold higher in normal cases compared with the healthy controls
Aanalysis showed that the area under the curve (AUC) for circulating microRNA
Pearson correlation (ρ) analysis showed that circulation of microRNA-1 correlated with serum levels of cardiac troponin I (CTnI) (ρ=0.24), creatinine (ρ =0.34), creatinine kinasemyocardial band (CK-MB) (ρ=0.31), and microRNA-221-3p was significantly
Summary
Methods: In the current study, 100 healthy controls (with no history of heart d disease) and 200 patients with MI were selected. Patients were divided into two te groups based on angiography results: normal (no significant artery stenosis) and primary percutaneous coronary intervention (primary PCI, significant artery stenosis). The levels of microRNA-1 and microRNA-221-3p were quantified p using real-time quantitative polymerase chain reaction. The correlation between levels of microRNAs and the common cardiac markers were analyzed e statistically
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