Abstract
BackgroundCacyBP/SIP (calcyclin binding protein/Siah-1 interacting protein) was originally discovered in Ehrlich ascities tumor cells but was later found also in many different tumors.MethodsTo better understand the function of CacyBP/SIP in carcinogenesis, we used immunohistochemistry, Western blotting, and RT-PCR assays to study the distribution and level of CacyBP/SIP in mammary tissues after tumor induction in rat with DMBA [(dimethylbenz[a]anthracene)]. Application of such a model allowed us to monitor changes in CacyBP/SIP level during development of breast cancer.ResultsWe found that both the protein and mRNA levels of CacyBP/SIP gradually increased in pathologically changed tissues and were highest in tumors taken 8 weeks after DMBA treatment. Similar changes as for CacyBP/SIP were detected in the level of β-catenin.ConclusionIncrease in CacyBP/SIP expression during development of breast cancer, observed early in the mammary tissues with only minimal pathological changes, might suggest an important role of this protein in the process of carcinogenesis.
Highlights
IntroductionCacyBP/SIP (calcyclin binding protein/Siah-1 interacting protein) was discovered in Ehrlich ascities tumor cells [1]
CacyBP/SIP was discovered in Ehrlich ascities tumor cells [1]
One group indicated that a lower level of CacyBP/SIP was present in the tumor tissue in comparison to the control one and, reduction in CacyBP/SIP expression was associated with poor prognosis of breast cancer patients [15]
Summary
CacyBP/SIP (calcyclin binding protein/Siah-1 interacting protein) was discovered in Ehrlich ascities tumor cells [1]. CacyBP/SIP seems to play a key role in the development of different cancers. Increased CacyBP/SIP expression is observed in renal cell carcinoma in which the up-regulation seems to be associated with suppression of cell growth and carcinogenesis [12]. It was reported that CacyBP/SIP could play an important role in colon cancer [13, 14]. These data suggest a possible involvement of CacyBP/SIP in carcinogenesis its precise role in this process still remains unclear. CacyBP/SIP (calcyclin binding protein/Siah interacting protein) was originally discovered in Ehrlich ascities tumor cells but was later found in many different tumors
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