Abstract
BackgroundSyntaxin4 (STX4) gene encodes the protein STX4, a member of soluble N-ethylmaleimide-sensitive factor attachment protein receptors protein, playing a vital role in cell invadopodium formation and invasion, which is associated with the malignant progression of various human cancers. However, the expression and prognostic significance of STX4 in kidney renal clear cell carcinoma (KIRC) remain to be investigated.MethodsIn this study, we collected the mRNA expression of STX4 in 535 KIRC patients from The Cancer Genome Atlasthrough the University of California Santa Cruz Xena database platform. Then we explored the expression of STX4 in KIRC, and the relationship with clinicopathological characteristics and prognostic value. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes function enrichment analyses were used to explore the potential mechanism of STX4 in KIRC. qRT-PCR analysis was performed toverify the above results with real world tissue specimens.ResultsThe results indicated that STX4 was up-expressed in KIRC, and were associated with higher histological grade, advanced stage, and poorer prognosis. Moreover, elevated STX4 expression is an independent risk factor for KIRC. qRT-PCR analysis showed that STX4 was significantly elevated in 10 paired of KIRC samples compared to normal samples. Functional enrichment analysis indicated that endo/exocytosis, autophagy, mTOR signaling pathway, and NOD-like receptor signaling pathway were enriched.ConclusionsIn summary, STX4 is constantly up-expressed in KIRC tissues, associated with a poor prognosis. We suggest that it can be an effective biomarker for the prognosis of KIRC and may be a novel therapeutic target in KIRC.
Highlights
Syntaxin4 (STX4) gene encodes the protein STX4, a member of soluble N-ethylmaleimide-sensitive factor attachment protein receptors protein, playing a vital role in cell invadopodium formation and invasion, which is associated with the malignant progression of various human cancers
After analyzing the expression levels of STX4 in 535 kidney renal clear cell carcinoma (KIRC) samples and 72 normal kidney samples, we found that the expression of STX4 in KIRC tissues was obviously higher compared with normal tissues (P
The expression of STX4 in KIRC tissues was significantly elevated by Quantitative reverse transcription polymerase chain reaction (qRT-PCR) result (P
Summary
Syntaxin (STX4) gene encodes the protein STX4, a member of soluble N-ethylmaleimide-sensitive factor attachment protein receptors protein, playing a vital role in cell invadopodium formation and invasion, which is associated with the malignant progression of various human cancers. The expression and prognostic significance of STX4 in kidney renal clear cell carcinoma (KIRC) remain to be investigated. Kidney renal clear cell carcinoma (KIRC) is the main histological subtype of the renal cell carcinoma (RCC), accounting for 80–90% of patients [1]. KIRC was one of the ten leading cancer types for the estimated new cancer cases and deaths in the United States, and it had caused about 14,770 new deaths according to cancer statistics data in 2019 [2]. More than half of KIRC patients are a symptomless and diagnosed incidentally on imaging [3]. It is urgent to study the carcinogenesis and progression of KIRC and explore new useful molecular markers for prognosis
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