Abstract
BackgroundReplication factor C (RFC) is closely related to tumor progression and metastasis. However, the functional significance of RFC2 in hepatocellular carcinoma remains unclear.Materials and methodsIn order to solve this problem, the expression of RFC2 in liver cancer patients was analyzed through ONCOMINE, UALCAN, Human Protein Atlas. Survival analysis was conducted using Kaplan–Meier plotter and GEPIA. GO and KEGG enrichment analyses were carried out. The protein–protein interaction (PPI) network was performed through Metascape. Western blotting, cell counting kit-8 and transwell assay were used to detect the effect of RFC2 on cell proliferation and migration.ResultsThe transcription and protein level of RFC2 in HCC were overexpressed, which was significantly related to the clinical individual cancer stage and pathological tumor grade of HCC patients. In addition, in patients with liver cancer, higher RFC2 expression was found to be significantly correlated with shorter OS and DFS. Furthermore, the function of RFC2 in liver cancer was DNA replication, and its main mechanism was the phase transition of the cell cycle. Biological experiments demonstrated that knockdown of RFC2 reduced the proliferation and migration of HCC cells.ConclusionRFC2 might promote the development of liver cancer, which might be achieved by regulating cell cycle and DNA replication. It could be used as a novel biomarker for the prognosis of liver cancer.
Highlights
Liver cancer is the second leading cause of cancer-related deaths worldwide [1]
In patients with liver cancer, higher Replication factor C (RFC2) expression was found to be significantly correlated with shorter overall survival (OS) and disease-free survival (DFS)
Biological experiments demonstrated that knockdown of RFC2 reduced the proliferation and migration of Hepatocellular carcinoma (HCC) cells
Summary
Liver cancer is the second leading cause of cancer-related deaths worldwide [1]. Hepatocellular carcinoma (HCC) is the main form of liver cancer (about 90%). Its main risk factors including chronic hepatitis B virus (HBV) and hepatitis C virus (HCV) infection, alcoholism, metabolic diseases, etc., which causes an inflammatory environment in the liver [3]. Long-term inflammatory stimulation leads to chromosomal instability, genetic and. The latest technology progress and method automation of RNA sequencing make full transcriptome analysis a reality. The fidelity of DNA replication is usually closely related to cancer progression. DNA damage repair and loss of checkpoint function can lead to genome instability. Replication factor C (RFC) is closely related to tumor progression and metastasis. The functional significance of RFC2 in hepatocellular carcinoma remains unclear
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