Up-regulated lncRNA-PVT1 expression in peripheral blood mononuclear cells of patients with coronary artery disease is correlated with decreased interleukin-10 production.

Peyman Nowrouzi-Sohrabi,Behnoosh Miladpour,Mahdi Namdar,Mehran Erfani,Mehdi Kalani,Atefeh Seghatoleslam,Alireza Hosseini Abgir,Mohammadjavad Tahmasebi,Shekoufeh Hatami

doi:10.1007/s11033-022-07181-y

Abstract

Plasmacytoma variant translocation 1 (PVT1) is a newly discovered long non-coding RNA, which has not been previously studied in the inflammatory responses of the peripheral blood mononuclear cells (PBMCs) of patients with coronary artery disease (CAD). This cross-sectional study was conducted on 15 CAD patients and 15 non-CAD (NCAD) individuals. The PVT1 expression was assessed in the PBMCs of the participants using a real-time polymerase chain reaction. Interleukin (IL)-10, IL-22, and matrix metalloproteinase-9 (MMP-9) were measured in the plasma and supernatant of cultured PBMCs in the presence or absence of lipopolysaccharide (LPS) using flow cytometry and enzyme-linked immunosorbent assay. An increased expression of PVT1 was observed in the untreated PBMCs of CAD patients, compared to the NCAD group. The PVT1 was significantly up-regulated after LPS treatment in the PBMCs of both groups. Plasma MMP-9 levels were found to be higher in CAD patients than in the control individuals. The level of IL-10 and IL-22 production by the non-treated PBMCs of CAD cases was significantly lower than the NCAD group. Overall, in the examined population, PVT1 expression was negatively correlated with IL-10 secretion. Moreover, the results showed a significant negative correlation between PVT1 expression and IL-10 production by untreated cells. The PVT1 expression augmented in the PBMCs of CAD patients, which could be associated with the decreased IL-10 generation by the PBMCs of these patients.

Highlights

Plasmacytoma variant translocation 1 (PVT1) is a newly discovered long non-coding RNA, and it has not been previously studied in the inflammatory responses of peripheral blood mononuclear cells (PBMCs) of patients with coronary artery disease (CAD)
An increased expression of PVT1 was observed in untreated PBMCs of CAD patients compared to the NCAD group
Plasma matrix metalloproteinase-9 (MMP-9) levels were found to be higher in CAD patients compared to the controls

Summary

Introduction

Plasmacytoma variant translocation 1 (PVT1) is a newly discovered long non-coding RNA (lncRNA), and it has not been previously studied in the inflammatory responses of peripheral blood mononuclear cells (PBMCs) of patients with coronary artery disease (CAD). IL-22, a member of the IL-10 related cytokine superfamily, has a dual nature in inflammation, which might present a pro-inflammatory or anti-inflammatory effect in order to modulate the tissue's immune responses [4]. All in all, it seems that IL-10 and IL-22 have beneficial effects in protecting against metabolic disorders, decreasing chronic inflammation and related complications [6]

Methods

Results

Conclusion