Unveiling the hub genes associated with aflatoxin B1-induced hepatotoxicity in chicken

Abstract

Aflatoxin B1 (AFB1), a ubiquitous and toxic mycotoxin in human food and animal feedstuff, can impair the function and health of some organs, especially the liver. However, the knowledge about the potential mechanisms of AFB1-induced hepatotoxicity in chickens is limited. Therefore, we analyzed the gene expression data of chicken embryo primary hepatocytes (CEPHs) treated with and without AFB1 at the dose of 0.1 μg/mL which were cultured at 37 °C in Medium 199 (Life Technologies, Shanghai, China) with 5.0% CO2 for 48 h. Totally 1,711 differentially expressed genes (DEGs) were identified, in which 1,170 and 541 genes were up- and down-regulated in AFB1-administrated CEPHs compared to the control, respectively. Biological process analysis suggested that these DEGs might take part in angiogenesis, cell adhesion, immune response, cell differentiation, inflammatory response, cell migration regulation, and blood coagulation. Signaling pathways analysis revealed that these DEGs were mainly linked to metabolic pathways, MAPK, TLR2, and actin cytoskeleton regulation pathways. Moreover, the hub genes, including GYS2, NR1H4, ALDH8A1, and ANGPTL3, might participate in AFB1-induced hepatotoxicity. Taken together, our study offers a new insight into the mechanisms of the AFB1-induced hepatotoxicity.