Year-end Sale % OFF 
Abstract
Although challenging, the generation of energy through the harvesting of electrons from biological fluids via fuel cells is a promising technology for wearable electronics. However, a current challenge is that neither a single molecular catalyst or an enzyme can completely oxidize fuels in biological fluids. With this issue in mind, herein, we report a hybrid bi-catalytic system allowing complete lactate oxidation by combining an organic catalyst, 4-amino-TEMPO (Amino-TEMPO), with a recombinant enzyme, oxalate decarboxylase (OxDc). This hybrid system was chosen due to the high oxidative catalytic activity of the molecular catalyst and the promiscuity of the recombinant enzyme to alternative substrates. The hybrid architecture enhanced the performance of the bioanode, enabling higher efficiency and improved stability of the energy-harvesting fuel cell. The hybrid system showed high catalytic activity, achieving a high and stable current density of 23.0 ± 1.5 mA cm−2 for lactate oxidation after 48 h of bulk electrolysis. Intermediate and product analysis by nuclear magnetic resonance (NMR) and gas chromatography (GC) coupled to a thermal conductivity detector (TCD) confirmed that the hybrid architecture acts in the multiples catabolic steps of lactate oxidation, enabling the complete oxidation of lactate to CO2 by collecting 12 electrons from the fuel molecule.
Accepted Version (
Free)
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
