Unveiling antimicrobial and anticancerous behavior of AuNPs and AgNPs moderated by rhizome extracts of Curcuma longa from diverse altitudes of Himalaya

Mamta Sharma,Pankaj Thakur,Enza Torino,Monika Monika,Rajesh Kumar,Reena V Saini

doi:10.1038/s41598-020-67673-4

Abstract

Conservative remedies have a gray history worldwide and these provide productive and pertinent tools to tackle ailments. Also, the high altitude areas of Indian Himalayas with their wealthy biodiversity anchorage around 2000 plant species. Ensuing study demonstrates the synthesis of Silver (Ag) and gold (Au) nanoparticles (NPs) and utilizes one of the medicaments Curcuma longa of Indian Himalayas collected from different altitudes. For the same, turmeric rhizome extracts have been prepared from the aforesaid medicament and its anticancer activity and antimicrobial potential have been evaluated. Formation of Ag and Au nanoparticles was realized via UV–Vis spectroscopy and transmission electron microscope (TEM) confirmed size of the NPs. Antibacterial activity has been checked against Bacillus subtilis and Escherichia coli. The anticancer prospective has been observed against A549 and PC3 cell lines of both Au and Ag NPs and the cytotoxicity on PC3 and A549 cell lines was assessed using MTT assay. Results revealed higher amount of biochemicals, antibacterial and anticancer activity in Ag and Au NPs synthesized from rhizome extract collected from highest altitude. For the first time impact of altitudinal variations on phytochemicals and nanoparticles has been reported which have significant effect on its antimicrobial and anticancerous activity.

Highlights

Abbreviations μg Micro gram μl Micro litre AgB Silver nanoparticles of extract of Bilaspur hill AgM Silver nanoparticles of extract of Mandi hill AgS Silver nanoparticles of extract of Shimla hill Au Gold AuB Gold nanoparticles of extract of Bilaspur hill AuM Gold nanoparticles of extract of Mandi hill AuS Gold nanoparticles of extract of Shimla hill DMSO Dimethyl sulfoxide EB Extract of Bilaspur hill shooliniuniversity.com
The ensuing endeavor present an evaluation of Curcuma longa and antimicrobial and anticancer activity of Ag and Au NPs synthesized from rhizome extract of p lant[20,34] collected from varied altitudes
Phenols, tannins, and terpenoids were found in maximum amount in rhizome extracts of higher altitude

Summary

Introduction

Abbreviations μg Micro gram μl Micro litre AgB Silver nanoparticles of extract of Bilaspur hill AgM Silver nanoparticles of extract of Mandi hill AgS Silver nanoparticles of extract of Shimla hill Au Gold AuB Gold nanoparticles of extract of Bilaspur hill AuM Gold nanoparticles of extract of Mandi hill AuS Gold nanoparticles of extract of Shimla hill DMSO Dimethyl sulfoxide EB Extract of Bilaspur hill shooliniuniversity.com. Plants behave differently in terms of biochemical aspects in order to better adapt to their environment. This broad range of environmental factors across altitude affects both, the chemical-composition and the survival of medicinal plants in such regions. The chemical synthesis of nanoparticles is a preferred method, which encompasses the use of toxic chemicals as reducing, capping and stabilizing agents that may lead to the formation of harmful and unsafe non environmental friendly byproducts. The need for eco-friendly and non-toxic techniques for the synthesis of nanoparticles lead to an increasing interest in the biological protocols which require no use of harmful chemicals and lack in the formation of toxic byproducts[1,2]. This plant contains flavonoids, proteins, phenolic compound (such as curcumin) saponins and several mineral elements among others which could reduce the metal ions to their n anoparticles[11,12]

Methods

Results

Discussion

Conclusion