Abstract
The allopathic herb Anisomeles malabarica (L.) R.Br ex Sims is a member of the Lamiaceae family of mints. This investigation aims to determine whether the acetone extract of Anisomeles malabarica (L.) R.Br ex Sims leaves exhibit in vitro antibacterial activity. The results of the phytochemical study showed that steroids, flavonoids, phenols, terpenoids, quinones, and saponin were present. TLC detected and separated potential chemical components present in the extract. The GCMS revealed the presence of volatile substances, and the LC-MS disclosed the existence of non-volatile substances based on the highest peak. The FT-IR spectrum data revealed the chemical compound's potential functional groups in the A.malabarica acetone extract. DPPH free radical scavenging showed that the acetone extract of conc. 40mg/ml, 60mg/ml, 80mg/ml and 100mg/ml possessed great antioxidant ability compared to standard. Escherichia coli, Bacillus subtilis, Bacillus cereus, and Klebsiella pneumonia were tested for antibacterial activity using the unpolished well diffusion technique. E. coli and B. subtilis were inhibited more effectively by the extract in conc.50μg/ml, 75μg/ml and 100μg/ml.
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