Abstract
An unusual structural component of plasmid pUC18 was observed in transformants of Escherichia coli strains. This molecule migrated faster than superhelical topoisomers in agarose gel electrophoresis and was difficult to stain with ethidium bromide. This unsual topology appeared independently of gyrase mutation, and the recA genotype may relate to accumulation of the plasmids conforming to the unusual topology.
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