Abstract
Bacteria realize the ability to communicate by production of quorum sensing (QS) molecules called autoinducers, which regulate the physiological activities in their ecological niches. The oral cavity could be a potential area for the presence of QS bacteria. In this study, we report the isolation of a QS bacterial isolate C10B from dentine caries. Preliminary screening using Chromobacterium violaceum CV026 biosensor showed that isolate C10B was able to produce N-acylhomoserine lactones (AHLs). This bacterium was further identified as a member of Burkholderia, an opportunistic pathogen. The isolated Burkholderia sp. was confirmed to produce N-hexanoyl-L-homoserine lactone (C6-HSL), N-octanoyl-L-homoserine lactone (C8-HSL), N-decanoyl-L-homoserine lactone (C10-HSL) and N-dodecanoyl-L-homoserine lactone (C12-HSL).
Highlights
Recent advances in the study of bacterial gene expression have shed light on the topic of cell-to-cell communication and community behavior that are critical for successful interactions between higher organisms
Most bacteria use quorum sensing (QS) to regulate numerous virulence determinants such as colonization and virulence factors indicating their pathogenicity that could cause a variety of human diseases [11] such as Burkholderia cepacia, an important pathogen in patients with cystic fibrosis often associated with bacteremia resulting in necrotizing pneumonia [3,12,13]
We investigated the presence of Burkholderia sp. from dentine caries samples and report the isolation of these bacteria that display QS properties
Summary
Recent advances in the study of bacterial gene expression have shed light on the topic of cell-to-cell communication and community behavior that are critical for successful interactions between higher organisms. Most bacteria use QS to regulate numerous virulence determinants such as colonization and virulence factors indicating their pathogenicity that could cause a variety of human diseases [11] such as Burkholderia cepacia, an important pathogen in patients with cystic fibrosis often associated with bacteremia resulting in necrotizing pneumonia [3,12,13]. The presence of such pathogenic human bacteria has triggered interest in isolating and studying them. We investigated the presence of Burkholderia sp. from dentine caries samples and report the isolation of these bacteria that display QS properties
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
