Abstract
Abstract Using spin trap methods, laser generated free radicals from cardiovascular tissue in high viscosity blood solution have been studied by EPR. The EPR spectra obtained are compared with spectra of free radicals produced by both cw and pulsed excimer laser irradiation. Nitroxide spin adduts produced by excimer laser irradiation show an unusual EPR hyperfine structure. In addition, the EPR spectra obtained by continuous wave (cw) laser irradiation are different from those obtained by excimer laser irradiation. The observed β-hydrogen hyperfine coupling for pulsed laser is approximately 1 Gauss smaller than those for cw laser irradiation. The present EPR results provide firm evidence that different free radical reaction intermediates are produced for each laser mode.
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