Abstract
Spores of the liverwort Sphaerocarpos donnellii Aust. were x-irradiated. Among 9557 plants five nutritionally deficient mutants could be identified. Three of them grew when supplemented with nicotinic acid. Crossing experiments demonstrated that the genes from these three mutants are non-allelic. Feeding experiments with compounds known to be intermediates in the nicotinic acid pathway in Neurospora were unsuccessful. Inasmuch as cinchomeronic acid (pyridine-3,4-decarboxylic acid) is considered to be a possible intermediate of nicotinic acid synthesis in E. coli , five related pyridine-decarboxylic acids were also tested. However, none of these compounds compensated for the nutritional deficiency.
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