Abstract
From children with growth retardation due to enteric disease, duodenal jejunal biopsy material was investigated. Using a modified Feulgen-reaction--acridine orange staining after acid hydrolysis according to Fukuda et al. (1986)--different DNA forms could be demonstrated in nuclei of cells of the lamina epithelialis mucosae by fluorescence microscopy. These clear cut forms of DNA by different fluorescence colours were only found in altered mucosa. The findings were discussed with regard to the possibility of mitotic disorders and cancerogenesis.
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