Abstract
BackgroundPolycystic ovary syndrome (PCOS) is not only a kind of common endocrine syndrome but also a metabolic disorder, which harms the reproductive system and the whole body metabolism of the PCOS patients worldwide. In this study, we aimed to investigate the differences in serum metabolic profiles of the patients with PCOS compared to the healthy controls.Material and methods31 PCOS patients and 31 matched healthy female controls were recruited in this study, the clinical characteristics data were recorded, the laboratory biochemical data were detected. Then, we utilized the metabolomics approach by UPLC-HRMS technology to study the serum metabolic changes between PCOS and controls.ResultsThe metabolomics analysis showed that there were 68 downregulated and 78 upregulated metabolites in PCOS patients serum compared to those in the controls. These metabolites mainly belong to triacylglycerols, glycerophosphocholines, acylcarnitines, diacylglycerols, peptides, amino acids, glycerophosphoethanolamines and fatty acid. Pathway analysis showed that these metabolites were enriched in pathways including glycerophospholipid metabolism, fatty acid degradation, fatty acid biosynthesis, ether lipid metabolism, etc. Diagnosis value assessed by ROC analysis showed that the changed metabolites, including Leu–Ala/Ile–Ala, 3-(4-Hydroxyphenyl) propionic acid, Ile–Val/Leu–Val, Gly–Val/Val–Gly, aspartic acid, DG(34:2)_DG(16:0/18:2), DG(34:1)_DG(16:0/18:1), Phe–Trp, DG(36:1)_DG(18:0/18:1), Leu–Leu/Leu–Ile, had higher AUC values, indicated a significant role in PCOS.ConclusionThe present study characterized the difference of serum metabolites and related pathway profiles in PCOS patients, this finding hopes to provide potential metabolic markers for the prognosis and diagnosis of this disease.
Highlights
Polycystic ovary syndrome (PCOS) is a kind of common endocrine syndrome and a metabolic disorder, which harms the reproductive system and the whole body metabolism of the PCOS patients worldwide
Pathway analysis showed that these metabolites were enriched in pathways including glycerophospholipid metabolism, fatty acid degradation, fatty acid biosynthesis, ether lipid metabolism, etc
The levels of fasting glucose, luteinizing hormone (LH), T, TG, low-density lipoprotein cholesterol (LDL-c), and LH/follicle-stimulating hormone (FSH) ratio were significantly higher in PCOS patients than those in controls, the levels of PRL, HDL-c were significantly lower in PCOS patients than those in controls (p value < 0.05)
Summary
Polycystic ovary syndrome (PCOS) is a kind of common endocrine syndrome and a metabolic disorder, which harms the reproductive system and the whole body metabolism of the PCOS patients worldwide. Due to the dysfunction of the ovary and metabolism, the incidence of negative consequences, such as gynecological cancer, hypertension, atherosclerosis, type 2 diabetes mellitus (T2DM), and cardiovascular disease (CVD), seem to be higher in PCOS women than those in normal populations [6,7,8]. In light of these risks, there is a strong need of reliable biochemical or molecular markers, which would enable to make the accurate diagnosis and effective therapy of PCOS
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