Abstract
The direct reaction between a radioiodine-labeled purified house dust allergen and human sera has been studied by precipitation of antigen-antibody complexes with cold ethanol. Antibody was found in sera of 8 of 16 nonallergic persons and 27 of 32 of those allergic to dust. The antibody was not found in significantly greater quantites in sera of patients who had received house dust injections than in sera of untreated patients. The antibody was destroyed by heating to 56° C. for 1 hour, by exposure to pH 4 for 2 hours, and by incubation with mercaptoethanol for 30 minutes. It was found predominately in the euglobulin fraction of serum. The reaction was inhibited by excess unlabeled house dust but not by a variety of other antigens. These antigen-antibody complexes vanished during prolonged incubation at 37° C., decaying in a reaction exhibiting first-order kinetics. The rate of decay was temperature dependent. It was inhibited by heavy metals, soybean trypsin inhibitor, some synthetic ester substrates of chymotrypsin, indol, and β indol acetic acid. It was not inhibited by 1.5 molar sodium chloride, ethylenediamine tetraacetic acid, phenol, iodoacetic acid- N-ethyl maleimide, p-mercuribenzoic acid dl tryptophane, glucuronolactone, salicylate, diisopropyl fluorophosphate, or epsilon-amino caproic acid.
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