Abstract
The H-4-II-E-C3 line has high aryl hydrocarbon hydroxylase (AHH) activity. It was also found to be very sensitive to benzo(a)pyrene (BP) toxicity, and a single-step selection procedure for isolating BP-resistant (BPr) clones was designed. All the BPr variants tested had reduced AHH activities under both inducing and noninducing conditions. As H-4-II-E-C3 was propagated, it gradually declined in AHH activity and increased in cloning efficiency in BP. The spontaneous rate of origin of the BPr variants was approximately 3 x 10(-5) events per cell generation. However, the variants gradually increased in AHH activity and decreased in cloning efficiency in BP as they were propagated. Partially resistant variants also generated, at an appreciable rate, cells with even greater degrees of resistance to BP. The instabilities of the wild-type and variant cultures suggest that the variants arose by an epigenetic mechanism or by changes in gene dosage, rather than by point mutations.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
