Abstract
Summary Five independent occurrences of Neurospora mutants requiring unsaturated fatty acids were found. Four of these were allelic and grew in the presence of either oleic, linoleic, or linolenic acid. The remaining mutant which was not allelic was partially blocked in the conversion of linoleic to linolenic acid. Biotin, aspartic acid, or pyridoxine did not affect the requirement for unsaturated fatty acids in these mutants. The mutants tested did not respond to arachidonic acid. The results were interpreted as indicating that the unsaturated fatty acids are synthesized through a route distinct from that of the saturated acids and that they are synthesized in the order of oleic to linoleic to linolenic acid.
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