Unsaturated dideoxy fluoro-ketopyranosyl nucleosides as new cytostatic agents: A convenient synthesis of 2,6-dideoxy-3-fluoro-4-keto-β- d-glucopyranosyl analogues of uracil, 5-fluorouracil, thymine, N4-benzoyl cytosine and N6-benzoyl adenine

Abstract

The β-protected nucleosides of uracil ( 2a), 5-fluorouracil ( 2b), thymine ( 2c), N 4-benzoyl cytosine ( 2d) and N 6-benzoyl adenine ( 2e) were synthesized by condensation of the peracetylated 3-deoxy-3-fluoro- d-glucopyranose ( 1) with the corresponding silylated bases. The nucleosides were deacetylated and several subsequent protection and deprotection steps afforded the partially acetylated analogues 6a– e. Selective iodination followed by hydrogenation gave the acetylated dideoxy analogues of uracil ( 8a), 5-fluorouracil ( 8b), thymine ( 8c), N 4-benzoyl cytosine ( 8d) and N 6-benzoyl adenine ( 8e), respectively. Finally, direct oxidation of the free hydroxyl group at the 4′-position of 8a– e, and simultaneous elimination reaction of the β-acetoxyl group, afforded the desired unsaturated 2,6-dideoxy-3-fluoro-4-keto-β- d-glucopyranosyl derivatives 9a– e. The new analogues were evaluated for antiviral and cytostatic activity. Compounds 9a– e were not active against a broad panel of DNA and RNA viruses at subtoxic concentrations. However, they were markedly cytostatic against a variety of tumor cell lines. The compounds should be regarded as potential new lead compounds to be further investigated for anticancer therapy.