Unresolved issues in pre-meiotic anther development.

Timothy Kelliher,Rachel L Egger,Virginia Walbot,Han Zhang

doi:10.3389/fpls.2014.00347

Timothy Kelliher, Rachel L Egger + Show 2 more

Open Access

https://doi.org/10.3389/fpls.2014.00347

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Abstract

Compared to the diversity of other floral organs, the steps in anther ontogeny, final cell types, and overall organ shape are remarkably conserved among Angiosperms. Defects in pre-meiotic anthers that alter cellular composition or function typically result in male-sterility. Given the ease of identifying male-sterile mutants, dozens of genes with key roles in early anther development have been identified and cloned in model species, ordered by time of action and spatiotemporal expression, and used to propose explanatory models for critical steps in cell fate specification. Despite rapid progress, fundamental issues in anther development remain unresolved, and it is unclear if insights from one species can be applied to others. Here we construct a comparison of Arabidopsis, rice, and maize immature anthers to pinpoint distinctions in developmental pace. We analyze the mechanisms by which archesporial (pre-meiotic) cells are specified distinct from the soma, discuss what constitutes meiotic preparation, and review what is known about the secondary parietal layer and its terminal periclinal division that generates the tapetal and middle layers. Finally, roles for small RNAs are examined, focusing on the grass-specific phasiRNAs.

Highlights

Successful anther development results in pollen dispersal
Most studies have focused on meiosis and pollen biogenesis, with the assumption that a simple lineage model explained how the typical four somatic wall layers with central archesporial (AR) cells arose from a stamen primordium (Figure 1A)
Patterning to achieve the four somatic wall layers and central pre-meiotic cells typical of anther lobes is summarized in Figure 1B, employing pale coloration to indicate initial specification, with cell types darkening as differentiated features emerge

Summary

Introduction

Successful anther development results in pollen dispersal. Steps required to achieve this are conveniently divided into three phases: organ patterning and initial cell differentiation, meiosis, and post-meiotic gametophyte development. Patterning to achieve the four somatic wall layers and central pre-meiotic cells typical of anther lobes is summarized, employing pale coloration to indicate initial specification, with cell types darkening as differentiated features emerge.

Results

Conclusion