Abstract
In order to understand the distribution of the main secondary metabolites found in Olea europaea L., eight different samples (olive leaf, stem, seed, fruit skin and pulp, as well as virgin olive oil, olive oil obtained from stoned and dehydrated fruits and olive seed oil) coming from a Picudo cv. olive tree were analyzed. All the experimental conditions were selected so as to assure the maximum coverage of the metabolome of the samples under study within a single run. The use of LC and GC with high resolution MS (through different ionization sources, ESI and APCI) and the annotation strategies within MetaboScape 3.0 software allowed the identification of around 150 compounds in the profiles, showing great complementarity between the evaluated methodologies. The identified metabolites belonged to different chemical classes: triterpenic acids and dialcohols, tocopherols, sterols, free fatty acids, and several sub-types of phenolic compounds. The suitability of each platform and polarity (negative and positive) to determine each family of metabolites was evaluated in-depth, finding, for instance, that LC-ESI-MS (+) was the most efficient choice to ionize phenolic acids, secoiridoids, flavonoids and lignans and LC-APCI-MS was very appropriate for pentacyclic triterpenic acids (MS (−)) and sterols and tocopherols (MS (+)). Afterwards, a semi-quantitative comparison of the selected matrices was carried out, establishing their typical features (e.g., fruit skin was pointed out as the matrix with the highest relative amounts of phenolic acids, triterpenic compounds and hydroxylated fatty acids, and seed oil was distinctive for its high relative levels of acetoxypinoresinol and tocopherols).
Highlights
Olive tree (Olea europaea L.), which has accompanied mankind since prehistoric times, has played a fundamental role in the economic, social, and cultural spheres of Mediterranean civilizations [1,2].Molecules 2018, 23, 2419; doi:10.3390/molecules23102419 www.mdpi.com/journal/moleculesNowadays, along with the consumption of olives and olive oil in the diet, the use of different olive fractions with therapeutic purposes is still deeply rooted in traditional medicine from many parts of the world
Olea europaea L. matrices were analyzed by GC-atmospheric pressure chemical ionization (APCI)-mass spectrometry (MS), LC-Electrospray ionization (ESI)-MS, and LC-APCI-MS, in order to create an analyte list with the m/z and retention time (Rt) of known molecules which could help to achieve the identification of as many compounds as possible in the selected samples
Regarding GC-APCI-MS signals, most of the compounds presented the m/z of the totally silylated molecule in their spectra, but MS signals corresponding to the loss of trimethylsilyl groups (-C3 H8 Si) as well as -OC3 H9 Si losses were commonly found
Summary
Olive tree (Olea europaea L.), which has accompanied mankind since prehistoric times, has played a fundamental role in the economic, social, and cultural spheres of Mediterranean civilizations [1,2].Molecules 2018, 23, 2419; doi:10.3390/molecules23102419 www.mdpi.com/journal/moleculesNowadays, along with the consumption of olives and olive oil in the diet, the use of different olive fractions with therapeutic purposes is still deeply rooted in traditional medicine from many parts of the world. The phytochemical characterization of these matrices has revealed the presence of a plethora of bioactive secondary metabolites belonging to different chemical classes, mainly phenolic and triterpenic compounds, tocopherols, sterols, and pigments [3,4]. Some of these phytonutrients found in olive fruits are transferred into the VOO [8,9,10] and are considered to be mainly responsible for the healthy benefits derived from its consumption [11,12]. Both effluents (olive mill wastewater) and solid wastes (olive pomace) containing phenolic compounds, organic acids, and lipids, are harmful to the environment
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
