Abstract
Cellular senescence plays a role in the onset of age-related pathologies and in the loss of tissue homeostasis. Natural compounds of food or plants exert an important antioxidant activity, counteracting the formation of harmful free radicals. In the presence of an intense stressing event, cells activate specific responses to counteract senescence or cell death. In the present paper, we aimed at evaluating the levels of expression of specific markers of senescence, in order to demonstrate that extracts from Myrtus Communis L. can prevent premature senescence in ADSCs exposed to oxidative stress. Cells were cultured in the presence of Myrtus extracts for 12–24 and 48 h and then incubated with H2O2 to induce senescence. We then evaluated the expression of senescence-related markers p16, p19, p21, p53, TERT, c-Myc, and the senescence-associated β-Galactoidase activity. Our results showed that pre-treatment with Myrtus extracts protects cells from premature senescence, by regulating the cell cycle, and inducing the expression of TERT and c-Myc. These findings suggest a potential application of these natural compounds in the prevention and treatment of various diseases, counteracting premature senescence and preserving tissue functions.
Highlights
Aging is a complex cellular response to stress
We have previously demonstrated that residues from myrtle liqueur production were able to counteract the appearance of a senescent phenotype in adipose-derived stem cells (ADSCs), exposed to H2 O2
mesenchymal stem cells (MSCs) are largely known for their ability to restore tissue function and differentiate into several phenotypes under appropriate stimuli [23], including osteogenic, chondrogenic, cardiogenic and adipogenic lineages [24,25,26,27]
Summary
Aging is a complex cellular response to stress. The accumulation of senescent cells in tissues contributes to the loss of homeostasis and the onset of age-related pathologies [1]. An altered balancing in ROS production is related to several diseases and cellular damages [2]. Natural molecules present in food or plants are largely known for their antioxidant activity, counteracting the formation of free radicals through the inhibition of lipid oxidation [3]. These small molecules can be used as an adjuvant treatment beside conventional therapy in cancer, due to their ability to reduce cancer
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
