Abstract
To understand the molecular mechanism underlying salt stress response in radish, iTRAQ-based proteomic analysis was conducted to investigate the differences in protein species abundance under different salt treatments. In total, 851, 706, and 685 differential abundance protein species (DAPS) were identified between CK vs. Na100, CK vs. Na200, and Na100 vs. Na200, respectively. Functional annotation analysis revealed that salt stress elicited complex proteomic alterations in radish roots involved in carbohydrate and energy metabolism, protein metabolism, signal transduction, transcription regulation, stress and defense and transport. Additionally, the expression levels of nine genes encoding DAPS were further verified using RT-qPCR. The integrative analysis of transcriptomic and proteomic data in conjunction with miRNAs was further performed to strengthen the understanding of radish response to salinity. The genes responsible for signal transduction, ROS scavenging and transport activities as well as several key miRNAs including miR171, miR395, and miR398 played crucial roles in salt stress response in radish. Based on these findings, a schematic genetic regulatory network of salt stress response was proposed. This study provided valuable insights into the molecular mechanism underlying salt stress response in radish roots and would facilitate developing effective strategies toward genetically engineered salt-tolerant radish and other root vegetable crops.
Highlights
Soil salinity/salinization is emerging as one of the typical problems that global crops confront at present, and more than one-fifth of total croplands suffer from salinization process (Flowers and Yeo, 1995)
Our study indicated that miR171a-targeted dehydrin ERD10, miR395a-targeted ATP sulfurylase 1 (APS1) and miR398-regulated CSD1 were able to mitigate salt-induced dehydration (Kovacs et al, 2008), nutritional disorders (Matsui et al, 2013) and oxidative stress (Jagadeeswaran et al, 2009), respectively. These findings provide a visualized insight into the molecular mechanism underlying salt stress response in radish
This large-scale proteomic study firstly provided a global view of proteome change under salt stress in radish roots
Summary
Soil salinity/salinization is emerging as one of the typical problems that global crops confront at present, and more than one-fifth of total croplands suffer from salinization process (Flowers and Yeo, 1995). Almost all major processes like seed germination, vegetative growth, flowering and fruit set are unfavorably disturbed, resulting in significant economic yield reduction and quality loss. Exposure to salinity can trigger severe disorders and disturbances in plants, including osmotic stress, ion toxicity/imbalance, reactive oxygen species (ROS) production. Proteomic Changes in Salt-Stressed Radish and other secondary damages. Plants have enacted strategies to survive under these salt-induced damages. The ROS-scavenging enzymes and antioxidants can protect cells from salinity-triggered oxidative damage in crops such as wheat (Mandhania et al, 2006) and potato (Aghaei et al, 2009). To reduce potential risks of salinity to crop yield and quality, it is of great importance to reveal the molecular mechanism underlying salt stress response in plants
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