Abstract
Abstract Objectives Myeloblastosis (MYB) proteins, recognized as crucial transcriptional regulators, play an integral role in orchestrating the range of plant colors. Materials and Methods Pitaya (Hylocereus spp.), a distinctive commercially cultivated fruit, stands apart due to its unique betalains, water-soluble nitrogen-enriched pigments that confer its vibrant hues. Although betalain biosynthesis has been extensively explored, the potential influence of co-activators that might disrupt pigment production remains relatively unexplored. Results In this study, we found that HuMYB9, an R2R3-MYB repressor, exhibited a remarkable decreasing during pitaya maturation. Sequence alignment analyses showed conserved R2 and R3 domains within the C-terminal of HuMYB9. HuMYB9’s regulatory activities were found to be nuclear localized and it interacted with specific elements within the promoters of HuDODA1, HuADH1, and HuCYP76AD1-1, thus influencing the transcriptional activities in vitro. HuMYB9 transiently downregulated the expression of key betalain biosynthetic genes with a corresponding effect on the levels of pitaya pulp betalains. Conclusions Our results suggest that HuMYB9 operates as a suppressor, specifically downregulating the expression of HuCYP76AD1-1, HuDODA1, and HuADH, thereby modulating betalain biosynthesis in pitaya. Collectively, our findings provide invaluable insights into the regulation of betalain accumulation in pitaya.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.